Biotin dctp

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August undefined, 2024

Web丁香通为您提供生物素11dCTP500商品详情介绍：价格：￥11，货号：2715-500nmol，CAS号：无，品牌：Lumiprobe，产地：美国，详见丁香通生物素11dCTP500商品详情页； Web1 – Set up labeling reactions. 1.1 For each labeling reaction, set up the PCR reaction mix as shown below: 1.2 Add 1 uL of 1 mM CF® dye dUTP to the reaction tube. Optional: for an unlabeled control reaction, add 1 uL of 1 mM dTTP instead of CF® dye dUTP. If using fluorescent dCTP, set up the reaction with 50 uM dCTP and 100 uM dTTP, then ...

APExBIO - Biotin-16-dCTP

WebThe biotin-modified dCTP analogs are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3' … WebWe also offer biotin aha-dUTP and aha-dCTP (B32766, B32772) and fluorescein aha-dUTP nucleotides, which can be used to generate nucleic acid probes that can be detected with streptavidin conjugates (Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices—Section 7.6, Molecular Probes avidin, streptavidin ... flovent help card

Biotin-11-dCTP AxisPharm

WebBiotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; … WebNov 21, 2024 · The biotin-dCTP was subsequently labeled by incubation with 0.1 µg/mL Streptavidin-Cy5 solution, for 30 min at 30 °C. Then, the slide was rinsed twice with Milli-Q water and dried out. WebA key-step of Hi-C experiments relies on efficient biotinylation of digested chromatin fragments that is performed via incorporation of Biotin-14-dCTP [2] or Biotin-14-dATP [3] by DNA polymerase I (Klenow fragment) (Fig.1). Figure 1: Efficient biotinylation of digested chromatin fragments is a key-step of Hi-C experiments. flovent glaxosmithkline coupon

Biotin-14-dCTP - Thermo Fisher Scientific

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WebA simple and sensitive methodology for mutation gene screening was developed. Click to read more. WebBiotin-16-dUTP is used as asubstrate for: Terminal Transferase. DNA polymerase I (holoenzyme and Klenow fragment) Taq DNA polymerase. Reverse transcriptase ( e.g., … greek brothersWebJul 17, 2024 · Briefly, 0.4 μM each outer primer, F3 and B3, 0.8 μM each loop primer, LF* and LB, 1.6 μM each inner primer, FIP* and BIP, 0.4 mM biotin-14-dCTP, 1 μl (8 U) of Bst DNA polymerase, 12.5 μl of 2× reaction buffer, and 1 μl of DNA template were added to a tube. The mixtures were heated at 64 °C for 1 h. greek brothers menu

"Web有些可以使用dATP、dTTP、dGTP、dCTP的酶，并不一定可以使用dUTP，具体需要参考该酶的产品说明书或自行测试一下。对于Taq酶催化的PCR反应，dUTP可以完全替代dTTP，也可以将 dUTP与其它dNTP等浓度添加到扩增体系中。 " - Biotin dctp

Biotin dctp

Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)* - AAT Bio

WebBuffer. H 2 O. Recommended Storage. -20°C or below. Other Name (s) N 4 -Biotin-OBEA-dCTP, N 4 -Biotin-dCTP. Application. Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies. Backbone. WebDescription: Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads.

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WebOverview. Biotin (vitamin B7) is a vitamin found in foods like eggs, milk, and bananas. Biotin deficiency can cause thinning of the hair and a rash on the face. Biotin is an … WebJan 17, 2024 · Biotin-labeled strands with incorporated EdU on beads were conjugated with 5-TAMRA-azide via the click reaction, and fluorescence intensity on beads was measured to quantify the amount of dCTP ...

WebBiotin-11-CTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace CTP in the in vitro transcription reaction for RNA labeling. Double-stranded DNA (i.e., linearized … WebDescription. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP. Biotin-14-dATP can be added to the 3' end of DNA using ...

WebNov 28, 2024 · Biotin side effects. Get emergency medical help if you have signs of an allergic reaction: hives; difficult breathing; swelling of your face, lips, tongue, or throat. … WebDescription: Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin …

WebDescription: Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin …

WebOptimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP. Please note: The optimal final concentration of Biotin-11-dCTP ... greek brother and sistersWebBiotin-16-dCTP is a modified deoxynucleoside triphosphate (dNTP), serving as a convenient tool for the enzymatic introduction of the biotin moiety into a nucleic acid target of interest. The biotin moiety exhibits a … flovent hfa 220 mcg canadaWebBiotin-14-dCTP is a patented dCTP analog with biotin attached at the N 4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of … greek brothers lincolnWebThe Township of Fawn Creek is located in Montgomery County, Kansas, United States. The place is catalogued as Civil by the U.S. Board on Geographic Names and its elevation … flovent hfa 110 mcg canadaWebBiotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; incorporation into DNA proceeds as with natural dCTP. … greek brothers 205WebDescription. RNase H，即核糖核酸酶H，是一种核糖核酸内切酶，可特异性地水解DNA-RNA结合链中RNA磷酸二酯键。. RNase H具有双二价金属离子催化机制，如Mg2+和Mn2+直接参与催化功能。. RNase H不能水解单链或双链DNA或RNA中的磷酸二酯键。. 本试剂盒可用于cDNA第二链合成前 ... greek brothers mythologyWebBiotin-14-dCTP is a dCTP analog with biotin attached at the N 4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, … greek brothers lincoln menu