Grf–gif chimeric protein
WebDec 15, 2024 · A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nat. Biotechnol. 38, 1274–1279 (2024). Article CAS Google Scholar ... WebBased on the observation that GRFs and GIFs interact to form a protein complex 15, we decided to evaluate the effect of a GRF-GIF chimeric protein. Here we show that …
Grf–gif chimeric protein
Did you know?
WebJun 10, 2013 · A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants A method to increase plant regeneration efficiency extends gene … We generated the Citrus and Vitis GRF–GIF chimeras by gene synthesis using the GRF and GIF homologs highlighted in Supplementary Fig. 1. We cloned the DNA fragments into pDONR by a Gateway BP reaction. We cloned the GRF–GIF chimeras into the pGWB14 binary vector by a Gateway LR reaction under … See more We performed all PCR cloning with Phusion High-Fidelity DNA Polymerase (New England BioLabs). We extracted RNA from spikes … See more Wheat transformation followed previously published protocols29. Briefly, we grew the different wheat and triticale cultivars in a greenhouse or a growth chamber under a long-day … See more We placed seeds of Carrizo citrange rootstock in water to soak and then peeled off the seed coats, making sure not to remove the … See more Rice transformation was performed following previously published protocols36. Briefly, we selected fresh rice seeds, dehusked them and … See more
WebApr 14, 2024 · Then, the miR396-GRF-GIF-SWI/SNF module played an essential role in GA signaling to control plant growth . In addition, the miR166e/calmodulin-like protein module was newly identified in our research, and the miR166 has been revealed to be involved in salt stress response and GA biosynthesis and catabolism . WebSep 2, 2024 · J.M.D. is co-inventor in patent US2024/0362601A1 that describes the use of chimeric GRF GIF proteins with enhanced effects on plant growth (Universidad Nacional de Rosario Consejo Nacional de Investigaciones Cientificas y Tecnicas). J.D. and J.M.D. are co-inventors in UC Davis patent application WO2024007284A2 that describes the use of …
WebNov 1, 2024 · Here, we show that expression of a chimeric protein including wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING FACTOR 1 (GIF1) dramatically increases … WebOct 13, 2024 · A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants, Nature Biotechnology (2024). DOI: 10.1038/s41587-020-0703-0 Journal information: Nature Biotechnology...
WebOct 12, 2024 · Dubcovsky and his colleagues looked at two other growth-promoting genes, GRF and GIF, that work together in young tissues or organs of plants ranging from moss to fruit trees. The team put these genes side-by-side, like a couple holding hands, before adding them to plant cells. “If you go to a dance, you need to find your partner,” …
WebOct 12, 2024 · A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM1, Tricoli DM2, Ercoli MF3, Hayta S4, Ronald P3, Palatnik JF5, Dubcovsky J1 Author information Affiliations 2 authors 1. Department of Plant Sciences, University of California, Davis, CA, USA. 1 author 2. horned caterpillar arizonaWebGenetic and molecular analysis revealed that the functions of GRFs in leaf growth and senescence can be uncoupled, demonstrating that the miR396-GRF-GIF network impinges on different stages of leaf development. Our results integrate the post-transcriptional control of the GRF transcription factors with the progression of leaf development. horned caterpillar mothWebJan 25, 2024 · Key message GRF–GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce. In addition, they enhance regeneration in multiple types of … horned carpenterWebA GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants.Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky … horned caterpillars identificationWebPrevious work showed that expressing a fusion of two interacting transcription factors, GROWTH-REGULATING FACTOR4 (GRF4) and GRF-INTERACTING FACTOR1 (GIF1), improved regeneration in wheat (Triticum aestivum). By overexpressing a chimeric fusion of ClGRF4 and ClGIF1, we achieved highly efficient transformation in watermelon. horned caterpillar poisonousWebJan 25, 2024 · GRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce. In addition, they enhance regeneration in multiple types of lettuce including butterheads, romaines, and crispheads. The ability of plants to regenerate in vitro has been exploited for use in tissue culture systems for plant ... horned caterpillarWebMar 1, 2024 · The GRF-GIF duo regulates the ATP-dependent DNA translocase SWI2/SNF2. (B) GIF-GRF chimeras boost plant regeneration. (Top) Plant tissues can be … horned cats