WebApr 15, 2011 · We used high success-rate DNA polymerase KOD FX to circumvent these PCR amplification problems. ... (25 mmol/L) 1.5 µL, dNTPs (10 mmol/L) 2.0 µL, primer (10 pmo1/µL) 1 µL, Taq polymerase (5 U/µL) 0.2 µL, and genomic DNA (50 ng/µL) 1 µL. The PCR parameters were 94°C for 5 min, 38 cycles of 94°C for 1 min, 38°C for 1 min, and 72°C for ... WebA 116 base-pair PCR product derived from a vWA locus was amplified by Taq, Pfu, or KOD DNA polymerase and compared by agarose gel electrophoresis and electrospray …

Multiplex gene editing of the Yarrowia lipolytica genome using the ...

WebKOD DNA Polymerase (formerly KOD HiFi DNA Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase (Nishioka 2001). KOD is a high fidelity thermostable DNA polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield for PCR applications (Takagi 1997). The enzyme's 3'→5' exonuclease-dependent ... http://www.bio-toyobo.cn/pdf/KFX-101CH.pdf healthy teas to drink at night

Newborn Screening for Spinal Muscular Atrophy: DNA Preparation …

WebSep 25, 2024 · The coding sequence (CDS) of DoMYB75 without a termination codon was amplified using the KOD FX High Success-rate DNA polymerase Kit (Toyobo Biotechnology Co. Ltd., Shanghai, China) and cloned ... WebThe KOD FX Neo enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3'→5' exonuclease activities, thus allowing for … WebSuch DNA polymerases are now commercially available. We compared the PCR performance of six direct PCR-type DNA polymerases (KOD FX, Mighty Amp, Hemo KlenTaq, Phusion Blood II, KAPA Blood, and BIOTAQ) in dried blood eluted from a filter paper with TE buffer. GoTaq Flexi was used as a standard DNA polymerase. PCR performance was … moulding stuttgart